Differential Gene Expression Analysis of Pure Cell Populations from Frozen Uterine Tissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
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چکیده
Laser Capture Microdissection (LCM) is an innovative technique which permits the rapid and reliable procurement of pure cell populations from heterogeneous tissue sections. After LCM, RNA isolated from pure cell populations was amplified to generate adequate amounts of antisense RNA (aRNA) for hybridization, and the differential gene expression profiles were analyzed between two experimental groups.
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Use of laser capture microdissection, cDNA microarrays, and tissue microarrays in advancing our understanding of prostate cancer.
One difficulty in studying epithelial tumors has been the inability to isolate pure samples for DNA and RNA analysis. Prostate cancer, with its infiltrative nature, is particularly challenging. The Combination of several new technologies should help overcome these hurdles. Laser capture microdissection uses a laser beam to achieve transfer of pure cell populations for isolation of DNA, RNA, and...
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